User Guide

• System Requirements

  • AutoGate for Mac
  • AutoGate for Windows
  • Software requirements
• Setup AutoGate

  • Install AutoGate on Windows
  • Install AutoGate on Mac
  • AutoGate - Possible Install Problems
  • Launch AutoGate
  • Launch AutoGate w/Matlab 2019a
  • Launch AutoGate w/Matlab 2020a
  • Launch AutoGate w/Matlab 2020a on Mac
  • Launch AutoGate w/Matlab 2020a on Windows
  • Launch AutoGate w/Matlab R2020a
  • Download AutoGate
• Start Experiment

  • New experiment
  • Existing experiment
  • Import from prior experiment
  • Demo expt - Allergy Diagnostic
  • Demo expt - bCell macrophage
  • Demo expt - bcell_EPP
  • Demo expt - bcell_N2D
• (Auto)Compensation

  • To do list
  • Describe experiment overview
  • Characterize Samples
  • Create, manage and assign keywords
  • Define stain sets and FMOs
  • Associate samples with stain sets
  • Run AutoComp
  • Set X/Y axes
  • Start/continue gating
  • Setup wizard preferences
  • Reuse prior sample and reagent info
• (Auto)Gating

  • Gating tree
  • View/Redo compensation
  • Grouping samples
  • Defining baseline sample
  • The plot begins
  • Plot editor preferences
  • Create gate & sub gates
  • Apply gates across samples
  • Manual gating
  • Path finder
  • View gating statistics
  • Publish gates to PPT, Word
  • Shrink/Restore Sample Size
  • Absolute Cell Count AKA True Count
  • UMAP Supervised Template (UST) - Cytof data
• Early Adopter version

  • AutoGate Bangalore - Mac
  • AutoGate Bangalore - Windows
• Release updates

  • Release notes 2019
  • Release notes 2018