AutoGate uses counting beads method to calcuate Absolute Cell Count. In this method, user is expected to specify # beads per ul, # ul used to re-suspend cells as part of the setup process, as below
Once the user completes the gating including beads, AutoGate instantly has the absolute cell count numbers. Users can check them in the tool tip on hovering over the gate (as below)
Also, the cell count is shown in the Stastics table (invoked by the table button from the gating tree window)
Absolute cell count in statistic table.