AutoGate will gate your clusters and subsets for you, and do it with far greater statistical rigor than you can. But, if at any time you want to DIY, AutoGate lets you gate by hand and mouse, much as you would in FlowJo.

Click the cluster you want to gate. To make it easier to see clearly, click on the zoom-in icon 

Go to the row of icons at the top of your plot window and click one of the geometic shapes – rectangular, polygon, or ellipse.

tools manual gate

Point your cursor on the cluster. A message will tell you which part(s) of the shape to click first. Put the shape in the subset you want to gate. Double-click. A window will appear, asking you to name the subset.

If you make a mistake at any point in the process, click the delete icon  and start over