It is helpful to declare the baseline sample within the group.
Right click on the baseline sample and choose Set as group’s sample control (baseline)
![](https://cytogenie.org/wp-content/uploads/2022/04/1.Define-baseline.png)
AutoGate will respond by noticing if a sample in other group has a similar treatment and thus asks this question
![](https://cytogenie.org/wp-content/uploads/2022/04/2.Prompt.png)
This sets the base line in both time groups as the sample with a value of “no allergen” (in this example) for the keyword treatment.
AutoGate responds by highlighting both samples in bold to indicate that they are base line sample.
![](https://cytogenie.org/wp-content/uploads/2022/04/3.Baselined.png)