In addition to AutoGate’s original semi-automatic gating, AutoGate in the last 3 years has acquired 5 fully automatic gating methods. Two methods are unsupervised: Exhaustive Projection Pursuit (EPP) and Uniform Manifold Approximation and Projection (UMAP). Three methods are supervised: multilayer perceptron (MLP) neural network based on MATLAB fitcnet; MLP based on Python TensorFlow and UMAP supervised templates.
Below is a comparison of the results with 7 publications based on average mass+distance similarity (QFMatch) and by overlap (F-measure)
|#||Publication||Cytometer||Year Published||Link to Publication|
T cells, dendritic
| Conventional |
B cells, macrophages