As mentioned in the pre-requisites for AutoGate, it requires Matlab to work. When you download AutoGate App from https://www.cytogenie.org, it will install Matlab R2020a/R2017a for you. If you prefer to install on your own, below are the instructions for running AutoGate with a recent version of Matlab – R2021b on Mac OS. Instructions for Windows OS is available here
1. Download Matlab R2021b runtime from https://www.mathworks.com/products/compiler/matlab-runtime.html
![](https://cytogenie.org/wp-content/uploads/2022/07/1.Runtime.png)
This is a once off install and doens’t have to be repeated when there’s a newer version of AutoGate.
1) It takes ~30 mins to complete the download (depending on your computer configuration and network speed)
2) Go to Downloads folder and extract the zip
![](https://cytogenie.org/wp-content/uploads/2022/07/2.Downloaded.png)
3) Open the MATLAB_Runtime_R2021b_Update_2_maci64.dmg file
![](https://cytogenie.org/wp-content/uploads/2022/07/3.Unzip_.png)
4) Open the Install file
![](https://cytogenie.org/wp-content/uploads/2022/07/4.InstallforMac.png)
5) As it’s downloaded from a trusted site, click Open
![](https://cytogenie.org/wp-content/uploads/2022/07/5.Alert_.png)
6) Provide your computer admin credentails when prompted
![](https://cytogenie.org/wp-content/uploads/2022/07/6.Pwd_.png)
7) It takes ~5 mins for the Matlab Install Wizard to show up. No changes / action required here or in any of the installation steps.
![](https://cytogenie.org/wp-content/uploads/2022/07/7.Installer_1-1024x616.png)
8) Click on the Next button in all the screens to complete the installation.
![](https://cytogenie.org/wp-content/uploads/2022/07/10.Installter_5-1024x607.png)
2. Download AutoGate2021b.app from here
1) Go to Downloads folder and right click > open on AutoGate2021b.tar to unzip
![](https://cytogenie.org/wp-content/uploads/2022/07/10.5.Unzip-tar.png)
2) Open AutoGate2021b.app.
![](https://cytogenie.org/wp-content/uploads/2022/07/11.AutoGate.app_-1024x115.png)
3) Though AutoGate2021b.app is from a trusted source, Mac computer displays this warning message. Click Cancel
![](https://cytogenie.org/wp-content/uploads/2022/07/12.AutoGate.app_alert.png)
4) Again open AutoGate2021b.app from the downloads folder. This time, click Open
![](https://cytogenie.org/wp-content/uploads/2022/07/13.AutoGate.app_Open.png)
5) AutoGate with Matlab 2021b will launch.
![](https://cytogenie.org/wp-content/uploads/2022/07/14.AutoGate_launched-1024x644.png)
Note: When there is a new version of AutoGate available, you will be alerted on launching AutoGate. Upon your confirmation, the updates will be automatically downloaded for you.
![](https://cytogenie.org/wp-content/uploads/2022/07/15.Update_available.png)