Ensure the appropriate X and Y axis are set.
To set the axes you click on the down arrow button next to the X and the Y axis labels to activate a drop down of choices.
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To change Y axis;
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Select the clusters either by;
- Clicking on the cluster
- Choosing from the cluster drop down from the tool bar
AutoGate responds by;
- Drawing a black border around the green cluster to signify user’s selection.
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Select other clusters, as required.
Once you specify the name and click OK, AutoGate responds by restricting the plot window to ONLY the cells/events within your chosen cluster.
You will also notice that the created gate is reflected in the Path finder and Gating tree.
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To create sub gates, use the same actions for the above gate(s)
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